Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EWSR1

Cell type

Cell type Class
Muscle
Cell type
DTC1
NA
NA

Attributes by original data submitter

Sample

source_name
Cell line
cell type
Derived from malignant pleural effusion of a patient with a chest wall CCS tumor
chip antibody
EWSR1 N (sc-48404, Santa Cruz)
treatment
untreated
cell line
DTC1 (CVCL_0J31)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked with formaldehyde, flash-frozen and processed for ChIP using Protein G beads. ChIP DNA was purified with AMPure XP beads. ChIP DNA libraries were prepared using the Illumina TruSeq ChIP library preparation kit (Illumina cat. no. IP-202-1012 or IP-202-1024) according to the manufacturer's instructions and 200-500 bp fragments selected using AMPureXP beads.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
19364651
Reads aligned (%)
79.0
Duplicates removed (%)
54.5
Number of peaks
3551 (qval < 1E-05)

hg19

Number of total reads
19364651
Reads aligned (%)
78.3
Duplicates removed (%)
55.7
Number of peaks
3560 (qval < 1E-05)

Base call quality data from DBCLS SRA